IGF-1


Our Unique EXPRESSION SYSTEM overcomes traditional manufacturing difficulties, making it possible to efficiently produce large-scale batches with non-animal origin raw materials.

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Use Ajinomoto IGF-1 To Enhance your Cell Culture Media

Ajinomoto's new IGF-1 represents a breakthrough in the way Insulin Growth Factors are manufactured. It offers:

  • 100X more active than insulin
  • Improved purity
  • Consistent quality in large batches

Our unique expression system overcomes traditional manufacturing difficulties, making it possible to efficiently produce large-scale batches with non-animal origin raw materials.

What's In It For You

By using Ajinomoto IGF-1, you can:

  • Reduce culture media costs
  • Take advantage of our manufacturing expertise & production experience
  • Buy directly from Ajinomoto and take advantage of our R&D expertise

Discover The Purer, More Cost-Efficient Growth Factor For Your Cell Cultures

 
Potential Comparative Charts
  Supplier A Supplier B Supplier C Ajinomoto
Source Recombinant
E. coli
 
Recombinant
E. coli
Recombinant
E. coli
Recombinant
C. glutamicum
Size .5, 5mg
 
.2, 1, 5, 10, 20mg .02, .1, 1mg 1mg, 10mg
Identity N-termical seq
analysis
 
M.W analysis N-termical seq
analysis
M.W. analysis
Form Solution (PBS) 0.3-1.0 mg/mL White Powder
Lypholized
Lypholized Solution (300mM CH3COONH3 pH5.5) .5mg/mL
 
Biological Activity ED50<3ng/mL
(MCF-7 cells)
 
ED50<10ng/mL
(L6 myoblasts)
ED50<1ng/mL
FDC-P1 cells
ED50 <1ng/mL
MCF-7cells
Endotoxin <0.5EU/ug
 
<0.1EU/ug <1EU/ug <0.1EU/ug
Purity >95%
(SDS-PAGE, HPLC)
>85% (HPLC) >97% HPLC
SDS-PAGE
>97% (SDS-PAGE
>85% (HPLC)
 

Usage Information

Preparation
Just prior to use, we recommend rapid thawing in a water bath at 25° C with gentle mixing.

Stability
Stability test is currently being performed. At this time, we have confirmed that no change in HPLC profile is detected after storage for nine months storage at 2° to 8° C, at -30° C, or at -80° C, respectively.

Media preparation
Direct addition to culture media is recommended. If pre-dilution is needed, PBS or mild acidic solutions (e.g. 100mM acetic acid) can also be used.

Detection
We confirmed that at least 0.5ng/mL could be quantitatively detected using a commercially available ELISA kit (e.g. R&D Systems, Inc., Cat# DG100).

Influence on media condition
IGF-1 is dissolved in c.300mM ammonium acetate, pH5.4. No effect on pH or osmolality of culture media should be expected because of large dilution in use (typically 1000 to 5000 times).

Questions?
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