Dipeptide/Oligopeptides Production

Ajinomoto recently developed a powerful biopharmaceutical technology for the production of small peptides. The process uses fermentation to produce an enzyme that subsequently bonds single amino acids into di-, tri-, and oligopeptides.

Ajinomoto's newly discovered enzyme system possesses a number of significant advantages over existing industrial methods of creating dipeptides/oligopeptides:

  • Faster reaction rates
  • Higher yields
  • Broad substrate specificity
  • Elimination of racemization separations
  • No need for the use of protected amino acid with costly deprotection steps

Ajinomoto's innovative and highly effective enzymation process can be used to produce a wide range of diverse short-length peptides. To see how our procees can help you improve amino acid stability or improve your cell culture operation please contact Ajinomoto sales.

 Dipeptide


Oligopeptides Production

  • Oligopeptides can also be synthesized using amino acid esters as acyl donors and peptides as nucleophiles.
  • Most peptides tested as nucleophiles attacked the amino acid methyl esters well, producing excellent yields of the corresponding oligopeptides
      (mM)
  L-Ala L-Ala-L-Ala 28.7
  L-Ala-L-Ala L-Ala-L-Ala-L-Ala 57.5
  L-Ala-L-Ala-L-Ala L-Ala-L-Ala-L-Ala-L-Ala 44.5
  L-Ala-L-Ala-L-Ala-L-Ala L-Ala-L-Ala-L-Ala-L-Ala-L-Ala 34.8
  L-Ala-L-Ala-L-Ala-L-Ala-L-Ala L-Ala-L-Ala-L-Ala-L-Ala-L-Ala-L-Ala 1.4*
L-Ala-OMe L-Ala-L-Gln L-Ala-L-Ala-L-Gln 15.2
  Gly-L-Ala L-Ala-Gly-L-Ala 25.9
  Gly-Gly L-Ala-Gly-Gly 41.7
  L-His-L-Ala L-Ala-L-His-L-Ala 55.9
  L-Leu-L-Ala L-Ala-L-Leu-L-Ala 48.3
  L-Phe-L-Ala L-Ala-L-Phe-L-Ala 49.7
  L-Phe-Gly L-Ala-L-Phe-Gly 43.7
  L-Ala-L-Tyr Gly-L-Ala-L-Tyr 1.7
Gly-OMe Gly-L-Gln Gly-Gly-L-Gln 7.2
  Gly-L-Tyr-L-Ala Gly-Gly-L-Tyr-L-Ala 44.2
L-Thr-OMe Gly-Gly L-Thr-Gly-Gly 83.0